logo

[세미나: 4월 28일(금), 오후 4시] 홍익대학교 바이오화학공학과, 김래현 교수

Title

Microphysiological systems for investigating the host-gut microbiota interactions

Speaker

홍익대학교 바이오화학공학과, 김래현 교수

EDUCATION

- 2009.09 ~ 2016.08 Ph.D. in Chemical and Biomolecular Engineering, University of Illinois at Urbana Champaign

- 2004.02 ~ 2006.02 M.S. in Chemical and Biological Engineering, Seoul National University

- 2000.02 ~ 2004.02 B.S. in Chemical and Biological Engineering, Seoul National University

RESEARCH EXPERIENCE

- 2020.01 ~ 2021.08 University of Washington, Seattle

- 2016.08 ~ 2019.12 University of North Carolina at Chapel Hill, Postdoctoral Research Associate working with Prof. Nancy L. Allbritton in both institutions

· Developing in vitro platforms for coculturing gut bacteria with primary intestinal epithelium

· Developing novel in vitro platforms for primary gastrointestinal epithelia and tissues

- 2009.09 ~ 2016.07 University of Illinois at Urbana Champaign, PhD Student under supervision of Prof. Mary Kraft

· Developed fluorescent sphingolipid precursor for visualizing cellular sphingolipid metabolites

· Assessed sphingomyelin metabolism by metabolic labeling

· Evaluated the role of sphingomyelinases on influenza virus infection cycle

· Investigated the effect of protein corona on cellular uptake of nanoparticles

- 2007.02 ~ 2009.07 Korea Institute of Science and Technology, Research Scientist, Hybrid Materials Research Center

· Examined the effect of synthetic polymers on biomimetic crystallization with liquid in-situ AFM

· Characterized mechanical properties of polyelectrolyte films and microcapsules with AFM

· Used conductive AFM to characterize CNT-polymer composite

- 2006.02 ~ 2007.02 Hynix Semiconductor (currently SK Hynix), Process Engineer (etching)

· Developed etch process for 66 nm DRAM fabrication

- 2004.03 ~ 2006.02 Seoul National University, Masters Student with Prof. Kookheon Char

· Investigated the effect of surface pre-treatment on the orientation of block copolymer thin films

- 2004.06 ~ 2004.09 Inter-University Semiconductor Research Center in Seoul National University, Research Assistant

· Developed processes for photolithography

| Date | Friday, April 28th, 2023

| Time | 16:00 ~ 

| Venue | 신소재공동연구소(131동) 1층 세미나실(제1세미나실)

[Abstract]

The human large intestine (also called the colon) forms a complex ecosystem with hundreds of microbes that have significant impacts on the health and disease states of the host. An intestinal microphysiological system that enables mimicking these host/gut microbial interactions can be useful for answering biological questions and testing gut microbial therapeutic interventions. In this presentation, the recent noteworthy efforts to develop simple and easy-to-use intestinal microphysiological systems are introduced. In particular, an in vitro model system that mimics the unique oxygen environment of the human large intestine is discussed. In this model, several obligate gut bacterial strains were successfully co-cultured with human primary colonic epithelial cells in physiological oxygen environments. Donor variability in host cell responses to bacterial coculture is also assessed. In addition, other microphysiological systems that recapitulate the important characteristics of the large intestines, such as the mucus layer, three-dimensional microstructures, and polarization of epithelial cells are discussed. Intestinal microphysiological systems will be of great interest in the biomedical and pharmaceutical industry and an important tool for understanding host-gut bacterial interactions and testing and screening therapeutic targets or toxic molecules.

| Host | 도준상 교수 (02-880-1605)