Seminar & Colloquium
[세미나: 4월 12일(금), 오후 4시] Ph.D. Ju Hee Ryu, Medicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology
Title
The influence of ligand spatial distribution on therapy using DNA origami nanostructure: CpG spacing for cancer vaccine
Speaker
Ph.D. Ju Hee Ryu, Medicinal Materials Research Center, Biomedical Research Division, Korea Institute of Science and Technology
* Education
- 2010. 9. ~ 2014. 8. Ph.D. School of Chemical and Biological Engineering, Seoul National University
- 2002. 3. ~ 2004. 2. M.S. School of Chemical Engineering, Seoul National University
- 1997. 3. ~ 2002. 2. B.S. School of Chemical Engineering, Seoul National University
* Experience
- 2015. 3. ~ present Senior/Principal Researcher, Biomedical Research Institute, Korea Institute of Science and
Technology
- 2016. 1. ~ 2019. 1. Principal Investigator, KIST-DFCI On-site Lab, Dana-Farber Cancer Institute
- 2016. 1. ~ 2019. 1. Visiting Scientist, Department of Biological Chemistry & Molecular Pharmacology, Harvard
Medical School and Department of Cancer Biology, Dana-Farber Cancer Institute
- 2007. 1. ~ 2015. 2. Researcher, Biomedical Research Institute, Korea Institute of Science and Technology
- 2004. 8. ~ 2007. 1. Researcher, Nanotechnology Research Team, Amorepacific corporation R&D center
| Date | Friday April 12th , 2024
| Time | 16:00 ~
| Venue | 신소재공동연구소 1층 세미나실
[Abstract]
The sophisticated interplay between various ligands and receptors significantly influences signal transduction pathways, ultimately affecting therapeutic outcomes. Recent research has shifted focus towards the receptors' ability to discern spatial arrangements of ligands, a key factor in the efficacy of therapeutic interventions. A prime example of this phenomenon is the impact of ligand spacing on nanoparticles. The spacing of multiple CpG oligonucleotides, in particular, has been identified as a determinant in Th1/Th2 immune polarization and in amplifying immune responses. This finding underscores the pivotal role of spatial characteristics in the presentation of multiple ligands on therapeutic nanoparticles and their subsequent effects on cellular functions.
Nonetheless, a prevalent challenge with current nanomaterials is their inability to ensure uniform spacing of CpG sites. They typically allow for the control of average spacing, which might lead to the simultaneous induction of Th1 and Th2 immune responses. To surmount this challenge, our study utilizes the precision of DNA origami nanostructures to achieve exact ligand spacing. We specifically explore the implications of varying CpG oligonucleotide spacing, which engage Toll-like receptors and function as danger signals in cancer vaccines.
In this study, we adopt a square block DNA origami approach to systematically evaluate the effects of varying CpG spacing, from 2.5 to 7 nm, in conjunction with antigens, in mouse melanoma models. We further assess the potential of augmenting therapeutic efficacy through the concurrent administration of anti-PD-L1. Our findings highlight the crucial role of precise ligand spatial distribution in modulating immune responses, offering novel insights for the design of more effective nanomedicine-based cancer vaccines.
| Host | 도준상 교수(02-880-1605)