Seminar & Colloquium
[세미나: 8월 17일(수), 오전 10시] Harvard Medical School, 오승은 박사
Title
Quantitative single-cell physiology of cytoplasmic density regulation using label-free microscopy
Speaker
오승은 박사, Department of Systems Biology, Harvard Medical School
Education
- 9/2003–6/2010 Ph.D. in Physics at Massachusetts Institute of Technology, Cambridge, MA
Advisor: Michael S. Feld, Ph.D.
- 3/1999–8/2002 B.S. in Physics at Seoul National University, Seoul, Korea
Summa cum Laude
Professional Experience
- 6/2017–Present Instructor, Department of Systems Biology, Harvard Medical School, Boston, MA
- 9/2010–Present Postdoctoral Fellow, Department of Systems Biology, Harvard Medical School, Boston, MA
Advisor: Marc W. Kirschner, Ph.D.
- 3/2004–8/2010 Graduate Research Assistant, G.R.Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA
Advisor: Michael S. Feld, Ph.D.
- 9/2002–6/2003 Research Assistant, Department of Physics, Seoul National University, Seoul, Korea
Advisor: Young Kuk, Ph.D.
| Date | Wednesday, August 17th, 2022
| Time | 10:00 ~
| Venue | https://snu-ac-kr.zoom.us/j/96354759793
[Abstract]
Stimulated Raman Scattering (SRS) microscopy visualizes the chemical composition of biological samples without any use of staining and has opened exciting opportunities in chemical, biological, and medical sciences. We developed Normalized Raman Imaging (NoRI), a computational enhancement that can transform SRS images to calibrated chemical analysis. NoRI reports the absolute concentration of the major chemical component at each pixel in a physical unit (mg/ml) by removing the effect of light scattering originating from samples. We demonstrated NoRI measurement of the local concentration of total protein, lipid, and nucleic acids in live or fixed cells and tissues with high 3-dimensional spatial resolution. The density of total protein or lipid is the combined result of the synthesis and degradation of macromolecules and the cell volume regulation. NoRI measurement provides evidence supporting that protein and lipid density are tightly regulated under homeostatic control with cell type-specificity, and change with physiological states and in diseases. We expect that NoRI will enable new paths towards understanding the physiological significance of protein and lipid concentrations.
| Host | 남기태 교수 (02-880-7094)