Seoul National Univ. DMSE
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Seminar & Colloquium

Seminar & Colloquium
[세미나: 8월 12일(금), 오후 2시] Michigan Technological University, 한상윤 교수

[세미나: 8월 12일(금), 오후 2시] Michigan Technological University, 한상윤 교수

 

Title

Myosin-Independent Mechanotransmission for Sensing Stiffness of Extracellular Matrix

 

Speaker

Prof. Sangyoon J. Han, Department of Biomedical Engineering, Michigan Technological University

 

Education

- 2007 - 2012 Ph.D., Mechanical Engineering, University of Washington, Seattle, WA

                  Dissertation title: Experimental and computational analysis of cell mechanics during spreading and migration

                  Advisor: Dr. Nathan J. Sniadecki, Professor in Mech. Engineering at University of Washington.

- 2002 - 2004 M.S., Mechanical Engineering, Seoul National University, Seoul, Korea (Advisor: Kunwoo Lee)

                  Thesis: Biomechanical study of artificial disc replacement in lumbar spine using finite element analysis

- 1998 - 2002 B.S., Mechanical Engineering, Seoul National University, Seoul, Korea

 

Professional Experience

- 2017 - present Michigan Technological University, Assistant Professor, Biomedical Engineering

- 2014 - 2017 University of Texas Southwestern Medical Center, Postdoctoral Scientist, Bioinformatics

                  Postdoc Advisor: Dr. Gaudenz Danuser, Chair of Bioinformatics Department, UT Southwestern, previously a Professor at Harvard Medical School, Cell Biology.

                  Research experience: Mechanobiology, Computer Vision, Inverse Problem, Light Microscopy, Single Particle Tracking, Convolutional Neural Networks, Time-series Analysis.

- 2012 - 2013 Harvard Medical School, Postdoctoral Scientist, Cell Biology

- 2007 - 2012 University of Washington, Research Assistant, Mechanical Engineering

- 2006 - 2007 Korea Institute of Industrial Technology (KITECH), Project Manager, Applied Robotics, Ansan, Korea

- 2004 - 2006 Daewoo Electronics Corp, Research Engineer, Digital Multimedia R&D Center, Gunpo, Korea

 

| Date | Friday, August 12th, 2022

| Time | 14:00 ~ 

| Venue | 131동 제1세미나실

 

[Abstract]

Mechanics of cellular microenvironment greatly influences physiology and pathology of cells and tissues. For example, increased tissue stiffness alone, without any chemical effects on it, can cause 

tumor progression. Mechanical sensing of the extracellular matrix (ECM) by cells occurs through cell-matrix adhesions that link the ECM to the cytoskeleton. As a mechanical anchor, cell-matrix 

adhesions transmit mechanical forces from the actin cytoskeleton to the ECM. At the same time, they transduce the force they “felt” into biochemical signals, cellular response to which lead to 

changes in a wide variety of cell functions including structural reinforcement. Cells transmit different levels of traction in response to the ECM stiffness, which is critical to induce different 

levels of signaling. One of the frequent destinations of signaling is non-muscle myosin II, which generates contractile force within F-actin cytoskeleton. In this talk, I will talk about a current 

understanding about this stiffness-dependent differential force transmission, its heavy dependence on nonmuscle myosin II, and our experimental findings about the differential force transmission 

independent of myosin II. The findings are based on the high-resolution traction microscopy, which we have recently developed and the actin fluorescence speckle microscopy. I will discuss a

new, actin polymerization-powered molecular clutch model that can explain the observed flow and traction. Together, our data suggest that initial ECM stiffness sensing event is regulated by actin 

polymerization-based retrograde flow, synergistically mediated by Arp2/3 and formin.

 

| Host | 도준상 교수 (02-880-1605)